The Centramate™ 500 S benchtop tangential flow filtration system provides biotechnology and biopharmaceutical drug developers & manufacturers with an ultra-compact portable unit for process development in ultrafiltration (UF) and microfiltration (MF) applications.

Designed for all applications requiring concentration and diafiltration of suspended micro-particulates (such as cell suspensions and lysates) or dissolved solutes (such as proteins or nucleic acids), the sanitary, self-venting, fully drainable design meets the demands of cGMP environments, with full traceability of process-wetted materials. Using the Centramate 500 S system for process development, time, risk, and process volumes are all minimized.

Features

• Compact Flowpath - the ultra-compact flow path of the Centramate 500 S system allows users to operate at volumes as low as 170mL (when using a single Centramate membrane cassette), enabling high concentration factors and minimizing operating volumes
• Scalable Process Technology - designed through experience, the Centramate 500 S system meets the requirements for cGMP equipment with a comprehensive qualification and documentation package, calibrated process instrumentation, and hygienic design
• Direct Flow Measurement - the magnetic inductive flowmeter provides direct flow measurement during operation, making system operation safe, accurate, and user- friendly
• High Yield and Product Recovery - the hygienic, completely drainable flow path of the Centramate 500 S system enables high product yield, optimum product recovery, and easy cleaning
• Safety in Operation - an adjustable pressure interlock provides product and operator safety in a robust and reliable TFF system
• Pressure Independent Flow - the four-piston diaphragm pump delivers constant flow even as the system pressure increases
• Data Logging Capability - data from the process instrumentation can be exported to a PC or laptop computer via the USB port on the Centramate 500 S system

The Centramate LV holder is fabricated from Stainless Steel (316L) with female luer fittings for use with 0.01 m² (0.1 ft²) and 0.02 m² (0.2 ft²) Centramate cassettes. The holder can accommodate up to 0.04 m² (0.4 ft²) of screen channel or 0.02 m² (0.2 ft²) of open channel Centramate cassettes at recommended cross flow rates. A range of luer fittings and valves are available as well as pressure gauges or transducers to set-up a complete TFF system.

The Centramate PE holder has a polyethylene manifold with ¼ in. NPT threaded ports sandwiched between 304 stainless steel outer plates for support. It accommodates up to 4 screen channel (3 suspended screen channel) 0.09 m² (1 ft²) cassettes. The use of spacers on the tie rods makes it easy to apply and tighten the nuts with minimal threading if only one cassette is installed. As additional cassettes are added, spacers can be removed. The Centramate PE holder is recommended for use in applications where metal contact must be avoided. A plastic, NPT threaded fitting kit, with either 2 or 3 pressure gauges is available. 

The Centramate stainless steel cassette holder (316L) comes with ½ in. sanitary flanges on feed and filtrate ports. The holder is supplied with surface and weld certification. Up to 5 screen channel (4 suspended screen channel) 0.09 m² (1 ft²) cassettes can be accommodated. Holder is supplied with both 1 in. and 2 in. spacers on the tie rods to simplify cassette installation and to apply the required torque. Spacers can be removed to compensate for the height difference as additional cassettes are added to the holder. Sanitary fitting kits with either 2 or 3 pressure gauges are available. 

Centramate suspended-screen cassettes with Omega membrane are perfect for laboratory scale and development applications in TFF. The cassettes are supplied with the same construction materials as used in the Centrasette membrane cassettes.

Centramate suspended-screen ultrafiltration cassettes with Omega membrane are perfect for laboratory scale and development applications in TFF. The cassettes are supplied with the same construction materials as used in the Centrasette membrane cassettes.

Applications developed on a Centramate suspended-screen ultrafiltration cassette with Omega membrane can be easily scaled up by adding cassettes to a Centramate holder or switching to the Centrasette cassette format with identical path length and channel format.

Pall's Omega polyethersulfone (PES) membranes offer high flux and selectivities. They have been specifically modified to minimize protein binding to the surface and interstitial structure of the membrane. This polymeric membrane is stable against biological and physical degradation due to the unique chemical properties of PES. 

Omega membranes are cast on a highly porous, non-woven polyolefin support. They have an anisotropic structure, a thin skin-like top layer with a highly porous underlying support. The structure of the skin determines the porosity and permeability characteristics of the membrane and can typically be cleaned quicker and easier than membranes with a uniform, sub-micron depth structure. This membrane is compatible with acids, bases and a variety of other cleaning agents. Omega membranes are available in a wide range of nominal molecular weight cutoffs (NMWC).

Delta regenerated cellulose membrane offers high flux and selectivity. The cellulose membrane cassette has been specifically developed to minimize protein binding to the surface and interstitial structure of the membrane. Inherently hydrophilic, this membrane shows low protein adsorption properties and is optimal for processes involving very hydrophilic proteins. Delta regenerated cellulose membrane shows low fouling characteristics, allowing a constant performance or flux during a production run. It is easy to clean and typically recovers normalized water permeability (NWP) by using only 0.8N saline solution.

Centramate microfiltration cassettes with Supor membrane are perfect for laboratory scale and development TFF applications. The cassettes are available in a range of channel formats to meet most application requirements. The cassettes are supplied with the same construction materials as used in the Centrasette membrane cassettes.

Applications developed on a Centramate cassette with Supor membrane can be easily scaled up by adding cassettes to a Centramate holder or by switching to the Centrasette cassette format with identical path length and channel format.